Acridine Orange

  • 3,6-Acridinediamine, N,N,N',N'-tetramethyl-, monohydrochloride
  • Acridine, 3,6-bis(dimethylamino)-, monohydrochloride
Formula
C17H19N3.HCl
Structure
Description
Brown solid.
Uses
Staining of cells. Diagnostic muscle biopsies.

Registry Numbers and Inventories.
CAS
65-61-2
EC (EINECS/ELINCS)
200-614-0
RTECS
AR7601000
RTECS class
Mutagen
Beilstein/Gmelin
3575587
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed
Japan ENCS (MITI)
Listed

Properties.
Formula
C17H19N3*ClH
Formula mass
301.82
Melting point, °C
284-287

Hazards and Protection.
Storage
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
Handling
Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with skin and eyes. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
Follow the OSHA respirator regulations found in 29CFR 1910.134 or European Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when necessary.
Small spills/leaks
Vacuum or sweep up material and place into a suitable disposal container. Wash area with soap and water. Clean up spills immediately, using the appropriate protective equipment. Avoid generating dusty conditions.
Stability
Stable under normal temperatures and pressures.
Incompatibilities
Strong oxidizing agents.
Decomposition
Hydrogen chloride, carbon monoxide, oxides of nitrogen, carbon dioxide.

Fire.
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. To extinguish fire use water spray, dry chemical, carbon dioxide, or chemical foam.

Health.
Exposure effects
May cause reproductive and fetal effects.
   Ingestion
May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
   Inhalation
May cause respiratory tract irritation.
   Skin
May cause skin irritation.
   Eyes
May cause eye irritation.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Get medical aid immediately.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists.
   Eyes
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately.

Transport.