Aniline Blue

  • Acid Blue 22
  • Benzenesulfonic acid, aminomethyl((4-((sulfophenyl)amino)phenyl)(4-((sulfophenyl)imino)- 2,5-cyclohexadien-1-ylidene)methyl)-, disodium salt
  • C.I. 42755
Formula
C32H25N3Na2O9S3
Structure
Description
Odorless dark purple powder
Uses
Histological stain.

Registry Numbers and Inventories.
CAS
28631-66-5
EC (EINECS/ELINCS)
249-113-9
RTECS
DB4958000
RTECS class
Mutagen
Beilstein/Gmelin
NA
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed
Japan ENCS (MITI)
Listed
Korea ECL
Listed

Properties.
Formula
C32H25N3Na2O9S3
Formula mass
737.72
Solubility in water
Soluble.

Hazards and Protection.
Storage
Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
Follow the OSHA respirator regulations found in 29CFR 1910.134 or European Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when necessary.
Small spills/leaks
Clean up spills immediately, using the appropriate protective equipment. Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal. Avoid generating dusty conditions. Provide ventilation.
Stability
Stable under normal temperatures and pressures.
Incompatibilities
Strong oxidizing agents.
Decomposition
Nitrogen oxides, carbon monoxide, oxides of sulfur, carbon dioxide, nitrogen.

Fire.
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. To extinguish fire use water spray, dry chemical, carbon dioxide, or appropriate foam.

Health.
Exposure effects
Prolonged or repeated skin contact may cause irritation. May cause methemoglobinemia, which is characterized by chocolate-brown colored blood, headache, weakness, dizziness, breath shortness, cyanosis, rapid heart rate, unconsciousness and possible death.
   Ingestion
May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
   Inhalation
May cause respiratory tract irritation.
   Skin
May cause skin irritation.
   Eyes
May cause eye irritation. This product contains an anionic dye. Similar dyes have not caused injury to the cornea or conjunctiva in documented exposure cases with human or rabbit eyes.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Get medical aid. Flush skin with plenty of soap and water.
   Eyes
Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.

Transport.