Blue tetrazolium

  • 3,3'-(3,3'-Dimethoxy)[1,1'-biphenyl]-4,4'-diyl)bis [2,5-diphenyl-2H-tetrazolium]dichloride; dimethoxyneotetrazolium
  • Ditetrazolium chloride
Formula
C40H32Cl2N8O2
Structure
Description
Solid
Uses
For research in seed germination, as stain for bacteria and molds, in histochemical studies, to demonstrate oxidation-reduction enzymes in normal and cancerous tissues.

Registry Numbers and Inventories.
CAS
1871-22-3
EC (EINECS/ELINCS)
217-488-8
S 22 24/25
Merck
12,9380
Beilstein/Gmelin
3894077
Beilstein Reference
4-26-00-01789
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed
Korea ECL
Listed

Properties.
Formula
C40H32Cl2N8O2
Formula mass
727.66
Melting point, °C
265 - 270
Solubility in water
Slightly soluble

Hazards and Protection.
Storage
Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.
Small spills/leaks
Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, using the appropriate protective equipment. Avoid generating dusty conditions. Provide ventilation.
Stability
Materials containing similar functional groups can decompose at elevated temperatures.
Incompatibilities
Strong oxidizers.
Decomposition
Chlorine, nitrogen oxides, irritating and toxic fumes and gases, nitrogen, chlorine.

Fire.
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. This material in sufficient quantity and reduced particle size is capable of creating a dust explosion. Extinguishing media: Use agent most appropriate to extinguish fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam.
Health
1
 
Flammability
0
 
 
Reactivity
0
 

Health.
Exposure effects
   Ingestion
May cause irritation of the digestive tract. The toxicological properties of this substance have not been fully investigated.
   Inhalation
May cause respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
   Skin
May cause skin irritation.
   Eyes
This product contains a cationic dye. Similar dyes have caused permanant injury to the cornea and conjunctiva in documented exposure cases with human or rabbit eyes.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes.
   Eyes
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately. SPEEDY ACTION IS CRITICAL!

Transport.
HS Code
2933 99 90