Cleland's reagent

  • Sputolysin
  • DL-1,4-Dithiothreitol
  • 1,4-Dithio-DL-threitol
  • DL-1,4-Dimercapto-2,3-dihydroxybutane
  • threo-2,3-Dihydroxy-1,4-butanedithiol
  • 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-
Formula
C4H10O2S2
Structure
Description
White powder.

Registry Numbers and Inventories.
CAS
27565-41-9
EC (EINECS/ELINCS)
248-531-9
RTECS
XO8576500
RTECS class
Mutagen
Beilstein/Gmelin
1719757
Beilstein Reference
3-01-00-02360
Canada DSL/NDSL
DSL

Properties.
Formula
C4H10O2S2
Formula mass
154.24
Melting point, °C
42
Boiling point, °C
125 - 130 (2 torr)
Vapor density (air=1)
5.3
Solubility in water
Soluble
pKa/pKb
9.29 (pKa)

Hazards and Protection.
Storage
Store in a cool, dry place. Keep container closed when not in use. Refrigerator (approx 4 C).
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.
Protection
Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to minimize contact with skin.
Respirators
Wear a NIOSH/MSHA or European Standard EN 149 approved full-facepiece airline respirator in the positive pressure mode with emergency escape provisions.
Small spills/leaks
Vacuum or sweep up material and place into a suitable disposal container.
Stability
No data.
Incompatibilities
Oxidizing agents, reducing agents, bases, alkali metals.
Decomposition
Carbon monoxide, oxides of sulfur, carbon dioxide, hydrogen sulfide.

Fire.
Flash Point,°C
> 110
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. To extinguish fire use water spray, dry chemical, carbon dioxide, or chemical foam.

Health.
Exposure effects
   Ingestion
May cause irritation of the digestive tract.
   Inhalation
May cause respiratory tract irritation.
   Skin
May cause skin irritation.
   Eyes
May cause eye irritation.

First aid
 
   Ingestion
Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
   Skin
Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes.
   Eyes
Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.

Transport.