Ethidium bromide

  • 2,7-Diamino-10-ethyl-9-phenylphenthridium bromide
Formula
C21H20N3.Br
Structure
Description
Dark read to purple solid.
Uses
Florescence dye for staining DNA.

Registry Numbers and Inventories.
CAS
1239-45-8
EC (EINECS/ELINCS)
214-984-6
EC Class
very toxic, irritant, mutagenic
R 46 22 26 36/38
RTECS
SF7950000
RTECS class
Tumorigen; Drug; Mutagen
UN (DOT)
2811
Merck
13,4751
Beilstein/Gmelin
1706118
Beilstein Reference
3-01-00-03032
Austrailia AICS
Listed
New Zealand
Listed
Korea ECL
Listed

Properties.
Formula
C21H20BrN3
Formula mass
394.32
Melting point, °C
261 - 264
Boiling point, °C
72 (0.1 torr)
Decomposition point, °C
260-262
Solubility in water
Soluble.
Refractive index
1.4575 (20 C)

Hazards and Protection.
Storage
Keep away from heat, sparks, and flame. Keep container closed when not in use. Store in a cool, dry, well-ventilated area away from incompatible substances.
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with skin and eyes. Keep container tightly closed. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to minimize contact with skin.
Respirators
Follow the OSHA respirator regulations found in 29CFR 1910.134 or European Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when necessary.
Small spills/leaks
Clean up spills immediately, using the appropriate protective equipment. Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Remove all sources of ignition. Provide ventilation.
Disposal code
9
Stability
Stable under normal temperatures and pressures.
Incompatibilities
Strong oxidizing agents.
Decomposition
Nitrogen oxides, carbon monoxide, carbon dioxide, hydrogen bromide.

Fire.
Flash Point,°C
>100
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with air. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Extinguishing media: For small fires, use water spray, dry chemical, carbon dioxide or chemical foam. Use agent most appropriate to extinguish fire.
Fire potential
Non-combustible, substance itself does not burn but may decompose upon heating to produce corrosive and/or toxic fumes.
Hazards
Contact with metals may evolve flammable hydrogen gas.
Combustion products
Fire may produce irritating, corrosive and/or toxic gases.
Health
2
 
Flammability
0
 
 
Reactivity
0
 

Health.
Exposure effects
May alter genetic material.
   Ingestion
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. The toxicological properties of this substance have not been fully investigated.
   Inhalation
Causes respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
   Skin
Causes skin irritation.
   Eyes
Causes eye irritation.

First aid
 
   Ingestion
Do NOT induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
   Inhalation
Remove from exposure to fresh air immediately. If breathing is difficult, give oxygen. Get medical aid. DO NOT use mouth-to-mouth respiration. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask.
   Skin
Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
   Eyes
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.

Transport.
UN number
2811
Response guide
Hazard class
6.1
Packing Group
I; II; III
 
HS Code
2933 99 90