HEPES

  • N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
  • 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
  • 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
Formula
C8H18N2O4S
Structure
Description
Liquid.
Uses
Biological buffer.

Registry Numbers and Inventories.
CAS
7365-45-9
EC (EINECS/ELINCS)
230-907-9
S 22 24/25
RTECS
TL6809000
RTECS class
Other
Merck
13,4672
Beilstein/Gmelin
883043
Beilstein Reference
5-23-02-00379
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed

Properties.
Formula
C8H18N2O4S
Formula mass
238.30
Melting point, °C
210 - 215
Density
1.448 g/cm3
Solubility in water
Very soluble
pKa/pKb
6.52 (pKb)

Hazards and Protection.
Storage
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Store protected from moisture.
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation. Keep from contact with moist air and steam.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.
Small spills/leaks
Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Provide ventilation.
Disposal code
3
Stability
Stable under normal temperatures and pressures. Hygroscopic: absorbs moisture or water from the air.
Incompatibilities
Moisture, strong oxidizing agents.
Decomposition
Nitrogen oxides, carbon monoxide, oxides of sulfur, irritating and toxic fumes and gases, carbon dioxide.

Fire.
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Extinguishing media: Use agent most appropriate to extinguish fire. Do NOT get water inside containers. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam.
Health
1
 
Flammability
0
 
 
Reactivity
0
 

Health.
Exposure effects
   Ingestion
May cause irritation of the digestive tract. The toxicological properties of this substance have not been fully investigated.
   Inhalation
May cause respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
   Skin
No information regarding skin irritation and other potential effects was found.
   Eyes
No information regarding eye irritation and other potential effects was found. Contact may cause transient eye irritation.

First aid
 
   Ingestion
Never give anything by mouth to an unconscious person. Get medical aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
   Skin
Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
   Eyes
Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.

Transport.
HS Code
2933 59 95