Methylene blue

  • Basic blue 9
  • 3,7-bis(Dimethylamino)-phenazathionium chloride
  • Calcozine blue zf
  • Chromosmon
  • Hidaco methylene blue salt free
  • Leather pure blue hb
Formula
C16H18ClN3S
Structure
Description
Dark green crystals or powder /Zinc free dye/. Slight odor.
Uses
Stain in bacteriology, reagent for several chemicals, in mixed indicators, as oxidation-reduction indicator, antimethemoglobinemic

Registry Numbers and Inventories.
CAS
61-73-4
EC (EINECS/ELINCS)
200-515-2
RTECS
SO5600000
RTECS class
Drug; Mutagen; Reproductive Effector; Human Data
Merck
12,6137
Beilstein/Gmelin
3599847
Beilstein Reference
4-27-00-05152
EPA OPP
39505
Swiss Giftliste 1
G-8473
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed
Japan ENCS (MITI)
Listed
Korea ECL
Listed

Properties.
Formula
C16H18N3S*Cl
Formula mass
319.86
Decomposition point, °C
190
Vapor density (air=1)
13
Density
1.757 g/cm3 (22 C)
Solubility in water
43,600 mg/L at 25 C
pKa/pKb
3.8 (pKa)

Hazards and Protection.
Storage
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Do not expose to air. Store protected from light. Store under an inert atmosphere.
Handling
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use only in a well ventilated area. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Store protected from light. Handle under an inert atmosphere. Store protected from air. Wash clothing before reuse.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.
Small spills/leaks
Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, using the appropriate protective equipment. Avoid generating dusty conditions. Provide ventilation. Place under an inert atmosphere.
Stability
Stable at room temperature in closed containers under normal storage and handling conditions.
Incompatibilities
Bases, reducing agents, air, dichromates, alkali iodides, oxidizing agents, caustic alkalis.
Decomposition
Hydrogen chloride, nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, sulfur oxides (SOx), including sulfur oxide and sulfur dioxide.

Fire.
Flash Point,°C
190
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. This material in sufficient quantity and reduced particle size is capable of creating a dust explosion. Extinguishing media: Use extinguishing media most appropriate for the surrounding fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam.
Health
2
 
Flammability
0
 
 
Reactivity
0
 

Health.
Poison_Class
3
Exposure effects
Effects may be delayed. Laboratory experiments have resulted in mutagenic effects. Repeated contact may cause corneal damage. May cause reproductive effects.
   Ingestion
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause hemolytic anemia. May be harmful if swallowed.
   Inhalation
Causes respiratory tract irritation. May cause methemoglobinemia, cyanosis, convulsions, tachycardia, dyspnea, and death. Can produce delayed pulmonary edema.
   Skin
Causes skin irritation.
   Eyes
Causes eye irritation. May cause chemical conjunctivitis.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
   Eyes
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.

Transport.