Ninhydrin

  • 2,2-Dihydroxy-1H-indene-1,3(2H)-dione
  • 1,2,3-Indantrione monohydrate
  • Indan-1,2,3-trione
Formula
C9H6O4
Structure
Description
White to light yellow crystals or powder.
Uses
Reagent for the detection of free amino and carboxyl groups in proteins and peptides, yielding a blue color under the proper conditions.

Registry Numbers and Inventories.
CAS
485-47-2
EC (EINECS/ELINCS)
207-618-1
EC Class
harmful, irritant
R 22 36/37/38
S 26 36/37/39
RTECS
NK5425000
RTECS class
Agricultural Chemical and Pesticide
UN (DOT)
2811
Merck
12,6646
Beilstein/Gmelin
1910963
Beilstein Reference
4-07-00-02786
Swiss Giftliste 1
G-2632
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed
Japan ENCS (MITI)
Listed
Korea ECL
Listed

Properties.
Formula
C9H6O4
Formula mass
178.15
Melting point, °C
250 - 258
Boiling point, °C
351
Vapor density (air=1)
6.16
Critical temperature
536
Critical pressure
55.2
Density
0.862 g/cm3 (20 C)
Solubility in water
Soluble
Partition coefficient, pKow
0.43

Hazards and Protection.
Storage
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.
Handling
Wash thoroughly after handling. Use only in a well ventilated area. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
Follow the OSHA respirator regulations found in 29CFR 1910.134 or European Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when necessary.
Small spills/leaks
Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal. Avoid generating dusty conditions. Provide ventilation.
Disposal code
3
Stability
Stable under normal temperatures and pressures.
Incompatibilities
Strong oxidizing agents.
Decomposition
Carbon monoxide, carbon dioxide.

Fire.
Autoignition, °C
241
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with air. To extinguish fire use water spray, dry chemical, carbon dioxide, or chemical foam.
Fire potential
This material is probably combustible.
Hazards
Dusts at sufficient concentrations can form explosive mixtures with air.
Combustion products
Fire may produce irritating, corrosive and/or toxic gases.
Health
1
 
Flammability
1
 
 
Reactivity
0
 

Health.
Poison_Class
3
Exposure effects
   Ingestion
May cause irritation of the digestive tract. May cause central nervous system depression.
   Inhalation
Inhalation of dust may cause respiratory tract irritation.
   Skin
Causes skin irritation. May cause reddening of the skin.
   Eyes
Causes eye irritation.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes.
   Eyes
Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately.

Transport.
UN number
2811
Response guide
Hazard class
6.1
Packing Group
I; II; III
 
HS Code
2914 40 90