(R,R)-Dithiothreitol

  • ,4-Dithiothreitol
  • 1,4-Dimercapto-2,3-butanediol, (2R,3R)-rel-
  • threo-2,3-Dihydroxy-1,4-butanedithiol
  • Cleland's reagent
  • Sputolysin
  • DTT
Formula
(C2H2OHSH)2
Structure
Description
Solid. Disagreeable odor.
Uses
Used to reduce disulfide bonds quantitatively and maintain monothiols in the reduced state. At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro.

Registry Numbers and Inventories.
CAS
3483-12-3
EC (EINECS/ELINCS)
222-468-7
EC Class
harmful, irritant
RTECS
EK1610000
RTECS class
Drug
UN (DOT)
3335
Merck
13,3412
Beilstein/Gmelin
1719757
Beilstein Reference
3-01-00-02360
Canada DSL/NDSL
DSL
US TSCA
Listed
Austrailia AICS
Listed
New Zealand
Listed

Properties.
Formula
C4H10O2S2
Formula mass
154.24
Melting point, °C
40 - 43
Boiling point, °C
125 - 130 (2 torr)
Solubility in water
Soluble
pKa/pKb
9.29 (pKa)

Hazards and Protection.
Storage
Store in a cool, dry place. Store in a tightly closed container.
Handling
Wash thoroughly after handling. Use only in a well ventilated area. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.
Protection
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators
Follow the OSHA respirator regulations found in 29CFR 1910.134 or European Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when necessary.
Small spills/leaks
Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal. Avoid generating dusty conditions.
Disposal code
3
Stability
Stable under normal temperatures and pressures.
Incompatibilities
Strong oxidizers.
Decomposition
Carbon monoxide, oxides of sulfur, carbon dioxide.

Fire.
Flash Point,°C
>110
Fire fighting
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with air. Extinguishing media: Use water spray, dry chemical, or carbon dioxide.

Health.
Exposure effects
   Ingestion
The toxicological properties of this substance have not been fully investigated. Organic sulfur compounds may cause headache, nausea, vomiting, and incoordination. Respiratory and muscular paralysis, kidney and liver abnormalities have also been reported.
   Inhalation
The toxicological properties of this substance have not been fully investigated.
   Skin
No information regarding skin irritation and other potential effects was found.
   Eyes
No information regarding eye irritation and other potential effects was found.

First aid
 
   Ingestion
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
   Inhalation
Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
   Skin
Get medical aid immediately. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes.
   Eyes
Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately.

Transport.
UN number
3335
Response guide
HS Code
2930 90 70